非洲猪瘟诊断技术研究进展
非洲猪瘟是猪的一种急性、高度接触性传染病,致死率可达100%。本文综述了非洲猪瘟病原学和血清学诊断技术研究进展,包括红细胞吸附试验(HAD)、聚合酶链反应技术(PCR)、环介导恒温扩增技术(LAMP)、荧光抗体技术(FAT)、酶联免疫吸附试验(ELISA)和胶体金快速免疫层析技术(GICA)等,并对各种方法的优缺点进行简要汇总分析,总结认为今后非洲猪瘟诊断的发展趋势在于不同诊断技术的联合应用。
Research Progress on Diagnostic Techniques for African Swine Fever
As a kind of acute and highly contagious disease,African swine fever(ASF)usually attacks pigs with a mortality rate of almost 100%. In this paper,the research progress of etiology and serological diagnostic technologies were reviewed,including hemoadsorption detection(HAD),polymerase chain reaction(PCR),loop-mediated isothermal amplification(LAMP),fluorescent antibody technique(FAT),enzyme-linked immunosorbent assay(ELISA)and gel-gold rapid immunochromatography(GICA),then,their advantages and disadvantages were briefly summarized and analyzed,therefore,it was believed that combined application of various diagnostic techniques would be the research direction in the future.
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