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2013—2017年我国小反刍兽疫病毒H基因分子演化特征

点击数:4563  时间:2019-09-17 00:00:00  来源: 中国动物检疫      作者: 刘春菊,盛琰翔,王清华,等

为研究我国小反刍兽疫病毒(PPRV)的分子流行特点,对20132017年我国37PPRV流行毒株进行血凝蛋白(H)基因序列测定和生物信息学分析。结果显示:37个毒株H基因核苷酸序列之间的遗传距离为0~0.007 7,变异分布在41个位点,H蛋白氨基酸序列之间的遗传距离为0~0.013 2,变异分布在24个位点。与15株代表毒株进行序列比对发现,20132017年我国37PPRV流行毒株H基因的13个位点发生了核苷酸序列突变,其中9个导致了氨基酸序列的改变。以最大似然法构建分子进化树,发现20132017年我国流行的37个毒株构成基因4系中一个独立的进化小分支。本研究阐明了20132017年我国PPRV H基因的分子演化特征,从而为该病控制和消灭策略的制定提供了数据支持。

Molecular Evolution Characteristics of H Gene of Peste Des Petits Ruminants Virus

in China During 2013 to 2017

In order to study molecular characteristics of peste des petits ruminants virusPPRVin China37 prevalent strains of PPRV collected from 2013 to 2017 were used for hemagglutininHgene sequencing and bioinformatics analysis. The results showed that the genetic distance among nucleotide sequences of H gene of these strains was 0 to 0.007 7involving 41 variable sitesand the genetic distance among amino acid sequences of H protein ranged from 0 to 0.013 2with 24 variable sites. Based on the sequence alignment against 15 reference strainsit was found that the mutation of nucleotide sequences took place in 13 variable sites of H gene of all these strainsof which9 nucleotide mutations resulted in the change of amino acid sequence. In additiona molecular evolutionary tree was constructed by maximum likelihoodit was found that the 37 studied strains could be grouped into a distinct clade in lineage IV. In shortthe molecular evolution characteristics of H gene of PPRV in China from 2013 to 2017 were stated in this paperwhich would provide data supports to formulate relevant strategies for control and eradication of the disease.

全文下载链接:http://kns.cnki.net/KCMS/detail/37.1246.S.20190903.1708.010.html



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